5x HOT FIREPol^® Probe qPCR Mix Plus (no ROX)

For in vitro use only

Description:

HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. 

HOT FIREPol^®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup

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