5x HOT FIREPol^® Multiplex qPCR Mix (ROX)

For in vitro use only

Description:

HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and ROX passive reference dye according to system requirements.

HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5′ flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.