Histology | NDIEMA HEALTHCARE LIMITED

Histology Reagents & Stains

DISPATCH TIME IN WEEKS:
2 to 4 weeks.

KIT DIMENSIONS:
2 x 50ml – 85x105x45 mm.
4 x 50ml – 170x105x45 mm.
4 x 250ml – 125x140X 125mm.

APPROXIMATE WEIGHT OF KIT:
2 x 50 ml – 266 gms.
4 x 50 ml – 493 gms.
4 x 250ml – 1.1 kg.

NO OF KITS IN SHIPPER:
2 x 50 ml – 92 kits.
4 x 50 ml – 46 kits.
4 x250 ml – 12 kits.

DIMENSION OF SHIPPER:
410x550x378 mm

APPROXIMATE WEIGHT OF SHIPPER:
2 x 50 ml – 26 kg.
4 x 50 ml – 24 kg.
4 x250 ml – 15 kg.

SAMPLES AVAILABILITY:
Yes.

PRODUCTION CAPACITY:
600 mn ml per annum.

PROCEDURE:

1. Spray Fixative on Slides. Drain excess. Dry in Air.
2. Pour cytochrome stain on fixed slide. Keep it for 60 sec.
3. Add 1 ml of diluted buffer.
4. Wash with Distilled water after 5-6 minutes.
5. Allow the smear to air dry.
6. Examine under oil immersion microscope.

RESULTS:
Neutrophils Purple Granules
Eosinophils Large Orange Granules
Lymphocytes Dark Blue Nuclei
Monocytes Kidney Shaped Smoky Blue
Basophils Large Blue Granules
Cellular Components
RBC Pinkish Red
Malaria Parasite Varying shades of Blue
Platelets Pale or Dark blue

CYTOCHROME STAIN WITH BUFFER

PROCEDURE:

Wright’s Stain With Buffer

WRIGHT STAIN WITH BUFFER

To detect intestinal protozoa

Principle:
Blood smears are prepared and fixed (using the Fixative). The cellular components when stained turn blue (basophilic) or orange granules (acidophilic). The color intensity can be varied by adjusting the staining time.

Kit components:
Reagent bottles
Kit insert.

Physical Appearance:
Wright stain: Clear ink blue coloured liquid without any particles.
Wright stain buffer: Clear colourless liquid without any particles.

Pack Size:
2 x 50 ml
4 x 50 ml
4 x 250 ml

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration
1. Colour change
2. Growth

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
2 x 50ml – 85x105x45 mm
4 x 50ml – 170x105x45 mm
4 x 250ml – 125x140X 125mm

Approximate weight of kit:
2 x 50 ml – 266 gms
4 x 50 ml – 493 gms
4 x 250ml – 1.1 kg

No of kits in shipper:
2 x 50 ml – 92 kits
4 x 50 ml – 46 kits
4 x250 ml – 12 kits

Dimension of shipper:
410x550x378 mm

Approximate weight of shipper:
2 x 50 ml – 26 kg
4 x 50 ml – 24 kg
4 x250 ml – 15 kg

Samples availability:
Yes

Production Capacity:
600 mn ml per annum.

PROCEDURE:
1. Spray Fixative on Slides. Drain excess. Dry in Air.
2. Pour Wright Stain on fixed slide. Keep it for 3 minutes.
3. Add Buffer. Keep it for 5 minutes.
4. Wash slide with distilled water.
5. Allow the smear to air dry.
6. Examine under oil immersion microscope.

RESULTS:
Neutrophils Dark Blue
Basophils Purple to Dark Blue
Lymphocytes Dark purple nuclei
Eosinophils Blue nuclei
Platelets Violet to Purple Granules

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
2 x 50ml – 85x105x45 mm
4 x 50ml – 170x105x45 mm
4 x 250ml – 125x140x125mm

Approximate weight of kit:
2 x 50 ml – 266 gms
4 x 50 ml – 493 gms
4 x 250ml – 1.1 kg

No of kits in shipper:
2 x 50 ml – 92 kits
4 x 50 ml – 46 kits
4 x250 ml – 12 kits

Dimension of shipper:
410x550x378 mm

Approximate weight of shipper:
2 x 50 ml – 26 kg
4 x 50 ml – 24 kg
4 x250 ml – 15 kg

Samples availability:

Yes

Production Capacity:
600 mn ml per annum.

PROCEDURE:
1. Spray Fixative on Slides. Drain excess. Dry in Air.
2. Dip fixed slide in Red stain for 5-10 seconds.
3. Wash slide with distilled water.
4. Dip this slide in Blue stain for 45-50 seconds.
5. Wash slide with distilled water.
6. Allow the smear to air dry.
7. Examine under oil immersion microscope.

RESULTS:
Leucocyte Nuclei Dark Blue
Leucocyte Cytoplasm Light Blue
RBC Pale Reddish
Malaria Parasite Varying shades of Blue
Platelets Pale or Dark Blue

Malaria Parasite Stain

MALARIA STAIN

Intended use:
Test for differential staining of cells for detection of parasites by Red and Blue stain.

Kit components:
Reagent bottles
Kit insert.

Physical Appearance:
Blue stain clear blue coloured liquid without any particles.
Red stain clear amber liquid without any particles.

Pack Size:
2 x 50 ml
4 x 50 ml
4 x 250 ml
Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration:
1. Colour change
2. Growth

Availability:
In Stock.

GIEMSA STAIN (READY TO USE)

GIEMSA STAIN (READY TO USE)

INTENDED USE:
Test for differential staining of cells for detection of parasites by Giemsa stain.

PRINCIPLE:
Smears are fixed using the Giemsa Fixative. Slides are immersed in Giemsa Reagent to differentially stain specific cellular components. The cellular components stain either basophilic (blue) or eosinophilic (orange granules). The color intensity can be varied by adjusting the staining time.

KIT COMPONENTS:
Reagent bottles.
Kit insert.

PHYSICAL APPEARANCE:
Giemsa stain clear purple coloured liquid without any particles.

PACK SIZE:
1 x 100ml
1 x 500 ml
STORAGE TEMPERATURE:
Room Temperature.

SHELF LIFE:
60 months

SIGNS OF DETERIORATION:
1.Colour change
2.Growth

AVAILABILITY:
In Stock.

DISPATCH TIME IN WEEKS:
2 to 4 weeks.

KIT DIMENSIONS:
1 X100ml – 48x120x48mm.
1 x 500ml- 100x200X 100mm.

APPROX WEIGHT OF KIT:
1 x 100 ml – 130 gms.
1 x 500ml – 620 gms.

NO OF KITS IN SHIPPER:
1 x 100 ml – 200 kits.
1 x 500 ml – 18 kits.

DIMENSION OF SHIPPER:
410x550x378 mm.

APPROXIMATE WEIGHT OF SHIPPER:
1 x 100 ml – 27 kg.
1x 500 ml – 13 kg.

SAMPLES AVAILABILITY:
Yes.

PRODUCTION CAPACITY:
600 mn ml per annum.

PROCEDURE:
1. Pour Fixative on Slides. Drain excess. Dry in Air.
2. Pour Ready-To-Use Giemsa stain on the smear keep for 10 minutes.

3. Wash slide with distilled water.
4. Allow the smear to air dry.
5. Examine under oil immersion microscope

RESULTS

Nuclei Blue

Basophilic cytoplasmic Blue

Cellular Components
Neutrophilic granules Purple
Eosinophilic granules Orange
Lymphocytes Dark Blue
Mast cell granules Deep blue-violet
Nucleoli Blue-violet
Red cells Pink
Cytoplasm of mature monocytes Grey blue

Procedure:
1. Prepare a thin smear on clean dry glass slide.
2. Allow it to dry and fix with gentle heat.
3. Stain with Albert’s Stain A for 3-5 minutes.
4. Drain the solution. Do not wash.
5. Apply Albert’s Stain B for 1 min.
6. Rinse with water. Blot dry.
7. Examine under oil immersion microscope.

RESULTS:
Metachromatic Granules: Bluish Black
Bacillary Body: Green or Bluish Green

ALBERT′S STAIN A & B

Intended use: Test for Metachromatic stain.

Principle:
The granules stain purple-black against the light green counterstained cytoplasm. This helps to distinguish Corynebacterium diphtheria from most of the short nonpathogenic diphtheroides which lack granules.

Kit components:
Reagent bottles.
Kit insert.

Physical Appearance:
Alberts stain ‘A’ clear peacock blue coloured liquid without any particles.
Alberts stain ‘B’ clear amber coloured liquid without any particles.

Pack Size:
2 x 50 ml
4 x 50 ml
4 x 250 ml

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration:
1. Colour change
2. Bacterial Growth

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
2 x 50ml – 85x105x45 mm
4 x 50ml- 170x105x45 mm
4 x 250ml – 125x140x125mm

Approximate weight of kit:
2 x 50 ml – 266 gms
4 x 50 ml – 493 gms
4 x 250ml – 1.1 kg

No. of kits in shipper:
2 x 50 ml – 92 kits
4 x 50 ml – 46 kits
4 x250 ml – 12 kits

Dimension of shipper:
410x550x378 mm

Approximate weight of shipper:
2 x 50 ml – 26 kg
4 x 50 ml – 24 kg
4 x250 ml – 15 kg

Samples availability:
Yes

Production Capacity:
600 mn ml per annum.

CARBOL FUSCHIN

CARBOL FUSCHIN

Intended Use: Staining Solution for Microbes

Principle:
This differential staining technique is useful for identification of the tubercle bacillus, other Mycobacteria and Nocardia, which depends on the chemical composition of the bacterial cell wall. Because of the difficulty in staining these organisms with ordinary dyes, basic dyes in the presence of controlled amounts of acid are used. Generally, heat must be applied during the staining procedure, or wetting agents must be used, to aid dye penetration. Organisms exhibiting the property of acid-fastness, once stained, are not easily decolourized by alcohol.

Kit components:
Reagent bottles.
Kit insert.

Physical Appearance:
Carbol Fuschin stain – Clear wine-red coloured liquid without any particles.

Pack Size:
1 x 100ml.
1 x 500 ml.

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration:
1. Colour change
2. Bacterial Growth

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
1 X100ml – 48x120x48mm
1 x 500ml – 100x200X 100mm

Approximate weight of kit:
1 x 100 ml – 130 gms
1 x 500ml – 620 kg

No. of kits in shipper:
1 x 100 ml – 200 kits
1 x 500 ml – 18 kits
Dimension of shipper: 410x550x378 mm

Approximate weight of shipper:
1 x 100 ml – 27kg
1x 500 ml – 13 kg

Samples availability:
Yes

Production Capacity:
600 mn ml per annum.

PROCEDURE
1. Prepare a smear on a clear, dry glass slide.
2. Allow it to air dry and fix with gentle heat.
3. Flood the smear with Carbol Fuchsin stain. Heat to steaming for 5 minutes with a low flame.
Do not boil the stain and do not permit drying of the smear.
4. Allow it to stand for 5 minutes without further heating.
5. Wash in the running tap water.
6. Decolourize with Acid Fast Decolourizer for 2 minutes or until no more stain comes off in the
washings. (If washing is not thorough, may get false positive results).
7. Wash with the tap water.
8. Counterstain for 30 seconds with Methylene Blue.
9. Wash with tap water. Dry in air. Examine under an oil immersion objective.

RESULTS
1.Acid fast organisms: Bright red
2 All other organisms: Blue

PROCEDURE
1.Prepare a smear on a clear, dry glass slide.

2.Allow it to air dry and fix with gentle heat.

3.Flood the slide with Crystal Violet.
4.Allow the stain to be in contact with the smear for 1-2 minutes.

5.Wash in slow-running water, just enough to remove excess of dye.
6.Flood the smear with Iodine, drain and flood again with Iodine for 1 minute.

7.Wash with decolourizer (alcohol) for about 5-15 seconds. Wash the slide to stop the action of decolorize.

8.Flood with safranin for 1 minute, wash very lightly.

9.Blot dry and examine under oil immersion objective.

RESULTS

Gram-positive organisms: Violet colored.
Gram-negative organisms: Pinkish red colored.

CRYSTAL VIOLET

CRYSTAL VIOLET

Intended Use: Staining Solution for Bacteria.

Principle:
Crystal Violet is used as a simple stain where it can render the organisms violet. Besides this it can also be used in the Gram staining for distinguishing between gram-positive and gram-negative organisms.

Kit components:
Reagent bottles.
Kit insert.
Physical Appearance:
Crystal violet colored liquid without any particles.

Pack Size:
1 x 100ml
1 x 500 ml
Storage Temperature:
Room Temperature.

Shelf life:
60 months.

Signs of Deterioration:
1. Colour change.
2. Bacterial Growth.

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
1 X100ml – 48x120x48mm
1 x 500ml – 100x200X 100mm

Approximate weight of kit:
1 x 100 ml – 130 gms
1 x 500ml – 620 kg

No. of kits in shipper:
1 x 100 ml – 200 kits
1 x 500 ml – 18 kits
Dimension of shipper: 410x550x378 mm

Approximate weight of shipper:
1 x 100 ml – 27kg
1x 500 ml – 13 kg

Samples availability:
Yes

Production Capacity:
600 mn ml per annum.

NEISSER′S METHYLENE BLUE

NEISSER′S METHYLENE BLUE

Staining Solution for Metachromatic staining.

Principle:
Well-developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractile bodies within the bacterial cytoplasm. With basic dye, they tend to stain more strongly than the rest of the bacterium, and with toluidine blue or methylene blue they stain metachromatically, a reddish purple colour. They are demonstrated most clearly by special methods, such as Albert’s and Neisser’s, which stain them dark purple but the remainder of the bacterium with a contrasting counterstain. The diphtheria bacillus gives its characteristic volutin-staining reactions best in a young culture (18-24 hours) on a blood or serum medium.

Kit components:
Reagent bottles
Kit insert.

Physical Appearance:
Neissers Methylene blue clear blue coloured liquid without any particles.

Pack Size:

1 x 100ml

1 x 500 ml

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration
1. Colour change
2. Growth

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
1 X100ml – 48x120x48mm
1 X 500ml – 100x200X 100mm

Approximate weight of kit:
1 x 100 ml – 130 gms
1 x 500ml – 620 gms

No of kits in shipper:
1 x 100 ml – 200 kits
1 x 500 ml – 18 kits

Dimension of shipper:
410 x 550 x 378 mm

Approximate weight of shipper:
1 x 100 ml – 27kg
1 x 500 ml – 13 kg

Samples availability:

Yes

Production Capacity:
600 mn ml per annum.

PROCEDURE
1. Prepare a thin smear on clean dry glass slide.
2. Allow it to dry and fix with gentle heat.
3. Pour Neisser’s Methylene Blue on fixed slide and keep it for 3 min.
4. Wash slide with Gram’s Iodine solution and keep Gram’s Iodine solution on slide for 1 min .
5. Wash slide with distilled water.
6. Pour Neutral Red solution, keep it for 3 min.
7. Wash with distilled water.
8. Allow the smear to air dry.

9.Examine under oil immersion microscope.

RESULTS
Metachromatic Granules Deep Blue
The Organisms Pink Colour

PROCEDURE:
1. Bring the section to slide using suitable adhering agent.
2.Slightly warm to melt the wax.
3.Remove the wax with Xylene by giving 3 times washing.
4. Remove the Xylene in 3 times washing with Dehydrant.
5. Dip the smear into distilled water for 3 minutes.

6. Dip the smear in Heamatoxylin (Harris) stain for 1min.
7. Wash with Distilled water.
8. Dip the smear in Eosin stain for 60 seconds.
9. Wash with distilled water for 15 seconds.
10. Rinse with Dehydrant and Xylene till it gets cleared.
11. Mount with glass cover using a drop of DPX.

RESULTS:
Nuclei Blue and Black
Cytoplasm Pink
Muscle Fibers Deep Red
RBCs Orange Red
Fibrin Deep Red

HEMATOXYLIN & EOSIN STAIN

Intended use:

HISTOPATHOLOGY STAIN.

Principle:
Alum acts as mordant and hematoxylin containing alum stains the nucleus light blue. This turns red in presence of acid, as differentiation is achieved by treating the tissue with acid solution. Bluing step converts the initial soluble red color within the nucleus to an insoluble blue color. The counterstaining is done by using eosin which imparts pink color to the cytoplasm.

Kit components:
Reagent bottles
Kit insert.

Physical Appearance:
Hematoxylin clear wine rad colored liquid without any particles.
Eosin clear reddish orange liquid without any particles.
Dehyrant clear colorless liquid without any particles.

Pack Size:

4 x 50 ml

4 x 250 ml

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration
1. Colour change
2. Growth

Availability:
In Stock.

Dispatch time in weeks:
2 to 4 weeks.

Kit dimensions:
4 x 50ml- 170x105x45 mm
4 x 250ml – 125x140X 125mm

Approximate weight of kit:
4 x 50 ml – 493 gms
4 x 250ml – 1.1 kg

No of kits in shipper:
4 x 50 ml – 46 kits
4 x250 ml – 12 kits

Dimension of shipper:
410x550x378 mm

Approximate weight of shipper:
4 x 50 ml – 24 kg
4 x250 ml – 15 kg

Samples availability:
Yes

Production Capacity:

600 mn ml per annum.