Hematology Reagents

 Hematology Reagents – a full line of dedicated reagents for automated cell-counters. … Our generic hematology reagents reach the level of quality, stability and shelf life of the original reagents, supplied by the instrument’s manufacturer. They will deliver good performance and the same accurate results.

5- PART REAGENTS & CONTROLS

Features :- 

  • Controls for 3 part and 5 part Hematology Analyzers.
  • Product basket includes Controls for all leading models of Mindray, Sysmex, Abbott, Horiba, Beckman Coulter, Siemens , Nihon Kohden & others.
  • Long shelf life of 6 months at 2-8o C.
  • Long Open Vial Stability of 30 days at 2-8oC.

Features :-

  • Controls for 3 part and 5 part Hematology Analyzers.
  • Product basket includes Controls for all leading models of Mindray, Sysmex, Abbott, Horiba, Beckman Coulter, Siemens , Nihon Kohden & others.
  • Long shelf life of 6 months at 2-8o C.
  • Long Open Vial Stability of 30 days at 2-8oC.

3- PART REAGENTS & CONTROLS

TOTAL IGE(IMMUNOGLOBULINE E)

Test Principle

 

  1. Total IgE from the sample binds with the anti-IgE on the solid phase.
  2. Unbound material is removed in a washing stage.
  3. Enzyme labeled anti-IgE is added which binds to total IgE.
  4. Unbound anti-human-IgE is removed in a washing stage.
  5. Substrate is added and the solution is incubated.
  6. Stop solution is added.
  7. The colour is measured with a photometer and the amount of total IgE is estimated using a standard curve.

Intended Use:
Test for qualitative detection of ABO antigens on human RBCs by immunoagglutination method.

Introduction:
The mouse monoclonal IgM Anti-A, Anti-B and Anti-AB antibodies are produced “in-vitro” as culture supernatant of selected hybridoma, obtained by the fusion of mouse antibody producing B-lymphocytes with mouse myeloma cells. Monoclonal sera ANTI-A, ANTI-B and ANTI-AB are specific IgM immunoglobulin which are directed against the human red blood cell antigens A, B and AB respectively. Monoclonal sera immunoglobulins are produced from individual cell line hence they are identical in their chemical structure and biological activity. The Rho (D) antigen is found on erythrocytes of approximately 95% of the Indian population. The terms “Rh” positive or “Rh” negative are understood to refer solely to the presence or absence of this antigen accordingly. Anti D (Rho) monoclonal IgM is used for the detection of the presence of Rho antigen on the red blood cells.

Method Principle:
Human red cells possessing A and/or B blood group antigen will be agglutinated by monoclonal sera directed towards the respective antigen (s), indicating positive test. Absence of agglutination cells with Anti-A, Anti-B and Anti-AB reagents is a negative test results and indicates the absence of the corresponding antigen. Human red cells possessing Rho D antigen will be agglutinated by monoclonal sera directed towards the respective antigen (s), indicating positive test. Absence of agglutination cells with ANTI-D (IgM), ANTI-D (IgG + IgM), Anti – D (IgG), Anti D (Rho IgG + IgM) reagents is a negative test results and indicates the absence of the corresponding antigen.

Pack Size:
3×10 ML

Kit components:
Anti ‘A’ Monoclonal – 1 bottle of 10 ml.
Anti ‘B’ Monoclonal – 1 bottle of 10 ml.
Anti ‘D’ Monoclonal (IgG+IgM) – 1 bottle of 10 ml.

Sample Type:
Whole Blood with Anticoagulant.

Titre:
1:512 for Anti-A and Anti-B.
1:256 for Anti-D (IgG+IgM).

Average Avidity:
< 10 seconds with whole blood.

Procedure:
Slide Test and Tube Test.

Reaction Time:
2 minutes.

Storage Temperature:
2- 8 0C.

Shelf life:
18 months.

Availability:
In Stock.

 

BLOOD GROUPING

Intended use:
HISTOPATHOLOGY STAIN.

Principle:
Alum acts as mordant and hematoxylin containing alum stains the nucleus light blue. This turns red in presence of acid, as differentiation is achieved by treating the tissue with acid solution. Bluing step converts the initial soluble red color within the nucleus to an insoluble blue color. The counterstaining is done by using eosin which imparts pink color to the cytoplasm.

Kit components:
Reagent bottles
Kit insert.

Physical Appearance:
Hematoxylin clear wine rad colored liquid without any particles.
Eosin clear reddish orange liquid without any particles.
Dehyrant clear colorless liquid without any particles.

Pack Size:

4 x 50 ml

4 x 250 ml

Storage Temperature:
Room Temperature.

Shelf life:
60 months

Signs of Deterioration
1. Colour change
2. Growth

Availability:
In Stock.