Cholesterol Esterase

Cholesterol Assay Kit ab65390 uses a simple method to quantify total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. It also includes an easy method to separate HDL and LDL / VLDL cholesterol.

In the cholesterol assay protocol, cholesterol oxidase acts on free cholesterol to produce a chemical which reacts with a probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase is used to hydrolyze cholesteryl ester into free cholesterol. Ifcholesterol esterase is included in the assay, total cholesterol is measured. If it is not included, free cholesterol is measured. The amount of cholesterol ester can be calculated by subtracting free cholesterol from total cholesterol.

Purpose	The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BSDL in Cell Lysate,Cell Culture Supernatant,Tissue Homogenate,Biological Fluids
Sample Type	Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Analytical Method	Quantitative
Detection Method	Colorimetric
Specificity	This assay has high sensitivity and excellent specificity for detection of Lipase, Bile Salt Dependent (BSDL). No significant cross-reactivity or interference between Lipase, Bile Salt Dependent (BSDL) and analogues was observed.
Cross-Reactivity (Details)	No significant cross-reactivity or interference between Lipase, Bile Salt Dependent (BSDL) and analogues was observed.
Sensitivity	0.058 ng/mL
Components	Pre-coated, ready to use 96-well strip plate Plate sealer for 96 wells Standard Diluent Assay Diluent A Assay Diluent B Stop Solution Standard Detection Reagent A Detection Reagent B TMB Substrate Wash Buffer (30 x concentrate) Instruction manual
Material not included	Microplate reader with 450 nm filter. Precision single or multi-channel pipettes and disposable tips. Eppendorf Tubes for diluting samples. Deionized or distilled water. Absorbent paper for blotting the microtiter plate. Container for Wash Solution