General description

Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas.^[1] GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.^[2]

Application

Glutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.^[4][3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.

Principle

GDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.